Fig. 5
From: High-resolution specificity profiling and off-target prediction for site-specific DNA recombinases
Off-target recombinase activity predicted by Rec-seq. a Cells were transfected with recombinase expression plasmid and an EGFP reporter plasmid containing candidate recombinase substrates flanking a poly-A terminator that blocks EGFP transcription. Tre and Brec1 activity on synthetic off-target substrates (b, c) and predicted endogenous human genomic pseudosites (d, e) was measured as the fraction of cells exhibiting EGFP fluorescence. The percentage of EGFP-positive cells shown is of transfected cells (determined by gating for the presence of co-transfected plasmid constitutively expressing mCherry) and 10,000 live events were recorded for each experiment. Data are represented as the mean (bars) of three independent biological replicates (dots). For d, e, significant differences (p ≤ 0.05) relative to no-enzyme control samples are indicated (Student's two-tailed t-test; colored asterisks). Source data are provided as a Source Data file
