Fig. 7
From: Red-shifting mutation of light-driven sodium-pump rhodopsin
The natural red-shifted NaR from J. seosinensis (JsNaR) without proline residue. a Pictures of the pellets of the E. coli cells expressing the KR2 wildtype, P219T, and JsNaR wildtype (left) and the absorption spectra of JsNaR wildtype (purple solid line), G216P (magenta solid line), and S247A mutants (indigo solid line). The absorption spectrum of KR2 wildtype and P219G mutant are shown by the magenta dotted line and cyan solid line, respectively. b Ion pump activity assays of the JsNaR wildtype. c The transient absorption spectra (left), time evolutions of the transient absorption change at specific wavelengths (middle) and the photocycle (right) of the JsNaR wildtype. The lifetimes and their standard deviations of the photo-intermediates in the photocycle of JsNaR are indicated. Source data are provided as a Source Data file. d The residues for colour regulating switches in microbial rhodopsins indicated by cyan spheres (left) and three types of switch working in various bacteria and archaea living in various environments in nature
