Fig. 3 | Nature Communications

Fig. 3

From: Loss of 5-methylcytosine alters the biogenesis of vault-derived small RNAs to coordinate epidermal differentiation

Fig. 3

Methylation-guided VT-RNA1.1 processing is altered in the absence of SRSF2. a, b Western blot for endogenous SRSF2 (a) and NSUN2 (b) in NSUN2-expressing (+/+, +/−) and -lacking (−/−) human fibroblasts (from two patients). Tubulin served as a loading control. c qRT-PCR measuring SRSF2-bound VTRNA1.1 normalised to the control (ctr; Rabbit serum conjugated with Dynabeads) after recovering the pulled down RNA in the SRSF2 immunoprecipitation. d Western blot for SRSF1 and SRSF2 in NSUN2−/− human fibroblasts infected with shRNAs (1.1, 1.6, 2.4, 2.5). Tubulin served as a loading control. e Fold-change (FC) of svRNA1 and 4 abundances after knock-down of SRSF1 and 2, relative to NSUN2−/− cells infected with the empty vector (e.V.). Shown are the pooled values using the two shRNA constructs shown in (d). Error bars indicate s.d. (n = 3-6 qRT-PCRs). **p < 0.01 students t-test. Source data are provided as a Source Data file

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