Fig. 5
SRSF2 is required for cell cycle and survival of undifferentiated cells. a Treatment regime and transfection of differentiating primary human keratinocytes. b Western blot detecting SRSF2 after treatment with Srsf2 siRNA. Tubulin (TUBB) serves as loading control. c Quantification of RNA expression levels of Ovol1, Vtrna1.1, Tgm, Krt10, and Ivl after 6 days of calcium-induced differentiation vs. untreated control (0 days). FC: Fold-change. Error bars indicate s.d. (n = 3 qRT-PCRs). **p < 0.01 two-way ANOVA. d, e Log2 RNA fold-change (FC) of differentiation markers (d) and cell cycle regulators (e) after 48 and 72 h of Srsf2 knock-down. Data shown as box plot with mean showing all data from minimum to maximum (n = 4). ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05 Two-way ANOVA. f Cell cycle distribution of primary HK transfected with the Srsf2 siRNA for 72 h. Error bars Data shown as box plot showing all data from minimum to maximum (n = 10 Flow sorts). ****p < 0.0001 Two-way ANOVA. g Light microscope images of HK transfected with a control (ctr) siRNA (upper panels) and a Srsf2 siRNA (lower panels) after 48 and 72 h in low calcium medium. Scale bar: 50 μm. h Small RNA qRT-PCR measuring the abundance of svRNA4 in primary HK transfected with the indicated siRNA constructs. Data shown as box plot with mean showing all data from minimum to maximum. Error bars represent s.d. (n = 4 qRT-PCRs). **p < 0.01 unpaired student’s t-test. i Illustration how levels of NSUN2, svRNA4, SRSF2, and OVOL1 change upon terminal differentiation in keratinocytes. Source data are provided as a Source Data file
