Fig. 4

SNF5 mimics the primary transcriptional effects of MYC inhibition. a Heatmaps displaying log₂-fold change (Log2FC) of active polymerases (compared to EGFP) in the promoter-proximal region and ±5Kb around the transcription start site (TSS), as determined by PRO-Seq. The two heatmaps are sorted in the same order; genes with increase in both OmoMYC and SNF5, genes with increase in OmoMYC only, and other active genes. Genes in each group are ranked by the OmoMYC FC near the TSS. b Heatmaps of genes showing changes in pausing index for OmoMYC and SNF5, compared to EGFP. Left panel: genes are ranked based on log₂-fold change of pausing index in OmoMYC, and the corresponding changes in SNF5 are shown on the right side. Right panel: genes are ranked based on log₂-fold change of pausing index in SNF5, and the corresponding changes in OmoMYC are shown on the right side. c Overlap of unique genes that had a change in pausing index upon OmoMYC expression with those that had a change following expression of SNF5 (FDR < 0.05). d Gene set enrichment analysis showing the distribution of genes that had an induced pause following SNF5 reintroduction against the ranked list of all genes with change in pausing index following OmoMYC expression (FDR < 0.05, genes ranked by log₂-fold change). e Gene set enrichment analysis, as in d, except for genes that had loss of pause following SNF5 reintroduction. f Overlap of genes that had increased pausing index (induced pause) for both OmoMYC and SNF5 (FDR < 0.0001). g Overlap of genes that had decreased pausing index (pause release) for both OmoMYC and SNF5 (FDR < 0.0001). h Overlap between the number of genes identified as MYC targets from ChIP-seq analysis (Fig. 3) that also had a resulting induced pause for each condition. n = 3 independent PRO-Seq experiments