Fig. 3

NV T-cells have a greater metabolic response upon activation. a ECAR and b OCR in NV, EM and CM T- cells upon stimulation with anti-CD3/CD28. c Fold ECAR change in NV, EM and CM T-cells upon activation. d NV, e EM and f CM ECAR ‘Pre’ and ‘Post’ activation taken from the measurements in the indicated boxes in Supplementary Fig. 3a. g Fold change in OCR in NV, EM and CM T-cells upon activation. h NV, i EM and j CM OCR ‘Pre’ and ‘Post’ activation taken from the measurements in the indicated boxes in Supplementary Fig. 3a. k ECAR/OCR ratio of ‘Pre’, ‘Peak’ and ‘Post’ activation kinetics of NV, EM and CM T-cells. Statistical analysis was performed using a non-matching one-way ANOVA with Tukey’s multiple comparison test (c, g, k). For non-parametric data, a Kruskal−Wallis test with Dunn’s multiple comparisons test was used. A one-sample t-test was also used comparing values to a theoretical value of 1 (c, g). A paired t-test was also used (d–f, h–j) whereby a Wilcoxon matched-pairs signed rank test was applied to non-parametric data. Data are representative of four experiments (a–k) and are expressed as mean ± SEM; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001