Fig. 2
From: A protein complex required for polar growth of rhizobial infection threads
Expression of VPY is tightly associated with rhizobial infection in root and nodule cells. a–i Images showing pVPY:GUS activity during rhizobial infection in root hair (a, e) and in nodules from primordia to mature zonation stages (b–d, f–i). Composite transgenic plants were used at different stages after inoculation with Rm1021-lacZ. a–e are whole mount of GUS and/or X-gal stained roots, f–i are longitudinal sections of GUS and X-gal stained nodule primordia/nodule. Blue colour indicates staining of GUS activity and magenta colour indicates staining of rhizobia. Arrowheads indicate GUS stained root hairs (a, e) and arrows indicate infection threads in root hairs (a, e) or nodule primordia (f–h)/nodule (i). v, central vasculature of the root; np, nodule primordium; n, nodule. Scale bars, 100 µm
