Fig. 6
From: A protein complex required for polar growth of rhizobial infection threads
VPY interacts with LIN during rhizobial infection thread formation. a–g Live cell confocal images showing a root hair undergoing infection in a sunn-2 composite plant containing pVPY: VPY-C-Venus, pLjUBQ1:N-Venus-LIN and pAtUBQ:DsRED as a transgenic marker. Bimolecular Fluorescence Complementation (BiFC) experiments were performed using N-Venus fused to LIN driven by pLjUBQ1 promoter and C-Venus fused to VPY driven by VPY promoter as in Fig. 5h. One representative image is shown out of seven imaged ongoing infection sites in two sunn-2 composite plants. Split Venus fluorescence was imaged 4 days after inoculation with Sm2011-CFP, in a root hair hosting an elongating infection thread. Venus fluorescence was associated notably to the tip of the growing infection thread. As in other root hairs, Venus fluorescence was also found associated to a number of other puncta in the nucleus vicinity or along the cytoplasmic bridge. DsRED, Venus and CFP were pseudo coloured in red, yellow and magenta respectively. Dashed white line box in a indicates the regions shown in (b–g). Arrows, infection thread growing tip; arrowheads, infection thread tip-associated Venus-labelled puncta. n, nucleus. Scale bars, 10 µm
