Fig. 2
From: Targeting lonidamine to mitochondria mitigates lung tumorigenesis and brain metastasis
LND and Mito-LND impact the activity of mitochondrial complexes, ROS generation and peroxiredoxin oxidation. a Effect of LND and Mito-LND on the activity of mitochondrial complexes I and II. H2030BrM3 cells were pretreated with LND or Mito-LND for 24 h. The mitochondrial complex I and II oxygen consumption rates (OCR) are plotted against concentration of LND or Mito-LND. Dashed lines represent the fitting curves used for determination of the IC50 values. b Effect of LND and Mito-LND on cellular ROS production, as measured by HPLC-based analyses of the oxidation of the hydroethidine (HE) probe. The scheme of HE oxidation is shown in the left panel, HPLC fluorescence traces recorded are shown in the middle panel and HPLC quantitative data in the right panel. c Effect of LND and Mito-LND on redox status of cytosolic (Prx1) and mitochondrial (Prx3) peroxiredoxins in A549 cells. Diagram depicting the various redox states of Prx1 and Prx3 is shown in the left panel. Representative immunoblots from cells treated with Mito-LND are shown in the middle panel, and the quantitative analyses of the redox status of Prx1 and Prx3 are shown in the right panels. Data are presented as the means ± SD. t test versus control: *p < 0.05, **p < 0.01
