Fig. 5
VWD patient sample results in mechanically weak microclots. a Overview of the strategy for comparing microclot mechanics between VWD sample and healthy donor sample. Healthy human washed platelets were added to VWD patient plasma and healthy donor plasma to form reconstituted VWD PRP (rVWD PRP) and reconstituted healthy PRP (rHealthy PRP). The mechanical properties of microclots formed using these reconstituted systems was compared with “Healthy PRP”, normal PRP isolated from healthy volunteer. b Phase contrast and fluorescence images of microclots formed using VWD patient sample and healthy donor sample. Measured fluorescence intensity (c), contractile force (d), stiffness (e) and volume (f) of the above two microclot groups. *P < 0.05, n > 10, each dot in box plot represents an independent experiment. All box plots with whiskers represent the data distribution based on five number summary (maximum, third quartile, median, first quartile, minimum). Statistical significance was determined by one-way analysis of variance (ANOVA)
