Fig. 2

Neuron-specific expression of TUBG1 mutations affects neuronal positioning a Coronal sections of E18.5 brains electroporated at E14.5 with either a control-empty vector, WT-TUBG1 or one of the four mutated forms of TUBG1 under the control of a neuron-specific DCX promoter and co-electroporated with a reporter construction encoding for GFP under the control of a neuron-specific NeuroD promoter (green). Sections were stained with DAPI (blue). CP, cortical plate; IZ, intermediate zone; VZ/SVZ, ventricular zone/subventricular zone. Scale bar 50 µm. b Quantification of fluorescent neurons in three different regions: up CP, down CP, and IZ for at least four electroporated embryos per group, Two-way ANOVA with Tukey’s multiple comparisons test, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, compared to control-empty vector or wild type TUBG1 overexpression. c Quantification of fluorescent neurons in the cortical plate. The image on the right shows the cortical plate divided in five equal sections (bins). Scale bar 50 µm. GFP-positive cells were counted in each bin for at least four embryos per condition, Two-way ANOVA with Tukey’s multiple comparisons test, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, black asterisks compared to control-empty vector and gray asterisks compared to TUBG1 wild type overexpression. d In utero electroporation of DCX-TUBG1-Y92C or WT together with GFP (green) in the ammonic neuroepithelium at E14.5. Images show coronal sections of the CA1 region of the hippocampus at P8 counterstained with CTIP2 marker (magenta). Scale bar 150 µm. Data are represented as mean ± s.e.m. Source data are provided in the Source Data file