Fig. 7
Tubg1Y92C/+ mice present with behavioral abnormalities a, b Repetitive behaviours. Distance travelled (a) and occurrences of rearing (b) numbered during 10 min of observation in a novel home-cage environment showing a significant increase for Tubg1Y92C/+ mice. c, d Novel object recognition (NOR) task. Mice were allowed to explore an identical pair of objects, and after 24 h, they were presented with the familiar object and a new object. The sniffing time in seconds during the 14 min of the presentation and test session are represented in box plots. (TIT 24 h, nose close to the object <1 cm, L) (c). The recognition index (RI) (d) was calculated as the percentage of time sniffing time novel object (NO) minus the time sniffing the familiar object (FO) divided by the total time of sniffing. In box plots center line represents the median, end of boxes 25th and 75th percentile and whiskers 10th and 90th percentiles. e Fear conditioning test. Graphs plot the freezing during the different sessions of the test. The 4 min of habituation (Hab1 and 2) and the 2 min post choc. The 6-min context session (Cont 1, 2, and 3) was run 24-h after conditioning. The 8-min cue session was performed 5-hours after the context session. A sequence of 2-min with no cue (pre cue1 and 2) and 2 min with light/auditory (cue 1 and 2) conditioning stimulus were repeated twice. Data are presented as mean ± s.e.m., n = 9 mice per genotype. One-way ANOVA (a, b, and d) or Two-way ANOVA (c, e, and f) *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided in the Source Data file. f. Representative EEG trace. Left panel: EEG recording before and after PTZ in WT mice without discharge activity. Right panel: Tubg1Y92C/+ mice epileptiform discharge events before PTZ and generalized tonicoclonic seizure after PTZ. L-C: left cortex; R-C: right cortex. n = 3 adult mice from each genotype
