Fig. 2 | Nature Communications

Fig. 2

From: Optimization of 4-1BB antibody for cancer immunotherapy by balancing agonistic strength with FcγR affinity

Fig. 2

FcγRs-crosslinking enhanced the activity of anti-4-1BB Abs. a Total splenocytes or b purified CD8+ T cells from B6 mice were stimulated with anti-CD3 and indicated anti-4-1BB Abs, 2 days later, the IFN-γ in culture medium was analyzed by CBA. c, d Purified CD8+ T cells from B6 mice were stimulated with anti-CD3 and indicated anti-4-1BB Abs when indicated single mouse FcγR-expressing B16-OVA cells were present, 2 days later, the IFN-γ in culture medium was analyzed by CBA. Mean ± SEM are shown. *p < 0.05, **p < 0.01 compared with control group or as indicated

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