Fig. 1
From: Ribosome biogenesis during cell cycle arrest fuels EMT in development and disease
Enhanced rRNA synthesis during EMT is independent of cell proliferation. a Proliferating (Control) and 48 h TGFβ-treated (TGFβ) NMuMG cells immunostained for E-cadherin, Vimentin, Snail1, DNA synthesis (EdU), rRNA synthesis (FUrd), and nascent peptide synthesis (AHA) (green). b FUrd, EdU, and AHA quantifications from a, P < 0.01, P < 0.001, P < 0.05. c Quantifications of FUrd and EdU in Py2T cells ± TGFβ, P < 0.04, P < 0.04. d Quantifications of FUrd and EdU in MCF7 cells ± hypoxia-induced EMT, P < 0.001, P < 0.05. e Immunostaining of FUrd (green), Snail2 (red), and DAPI (blue) in delaminating/migrating neural crest cells (white arrows) of the chick neural tube. Representative inset of the neural crest delaminating region is included. f Immunostaining of EdU (green), Snail2 (red), and DAPI (blue) in the chick neural tube with a representative inset of the neural crest delaminating region. g Immunostaining of Sox10 (green), DNA synthesis (BrdU, red), and rRNA synthesis (EU, cyan) in mouse E9.0 neural tube. Migrating neural crest cells are indicated with white arrows. h Illustration of neural crest delamination/migration showing patterns of detected rRNA (green, left half) and DNA synthesis (red, right half). i Immunostaining of E-cadherin (green), Vimentin (green), colocalization of RNA synthesis (FUrd, green) with DNA synthesis (EdU, red), and brightfield images at 27, 48, 96 h ± TGFβ in NMuMG cells. j Illustration of quantified rRNA/DNA synthesis (FUrd/EdU) time course from (i) in Control (proliferation) and TGFβ (EMT) conditions. Red and green shapes depict FUrd (P < 0.01) and EdU (P < 0.02) quantifications. All error bars ± SE, n = 3. Asterisk denotes t-test significance at P ≤ 0.05. Scale bar for all images = 50 µm
