Fig. 5
From: Local membrane charge regulates β2 adrenergic receptor coupling to Gi3
Ca2+ and Mg2+ facilitate Gi3 coupling in negatively charged bilayers a The effect of CaCl2 and MgCl2 concentration on mB−β2AR fluorescence in DOPS and DOPG nanodisc bilayers was examined in the presence and absence of Gi3. Epinephrine was included (30 μM) or omitted (APO). EC50 is mean ± s.e.m. b The effect of G protein concentration (Gi3 and Gs) on mB−β2AR fluorescence ± 3 mM CaCl2 was examined in DOPS nanodiscs in the absence (APO) and in the presence (EPI) of epinephrine (30 μM). c The effect of Gi3 on mB−β2AR fluorescence ± 3 mM CaCl2 was examined in DOPE, DOPC, and DOPG nanodisc bilayers in the presence of 30 μM epinephrine (EPI). d The effect of Gi2 concentration on mB−β2AR fluorescence ± 3 mM CaCl2 was examined in DOPS nanodisc bilayers in the presence of 30 μM epinephrine (EPI). a–d mB−β2AR concentration is 100 nM. The net charge of the phospholipid molecule is indicated in parentheses. Data are mean ± s.e.m. of three independent experiments. Source data are provided in the Source Data File
