Fig. 3 | Nature Communications

Fig. 3

From: Circulating miR-103a-3p contributes to angiotensin II-induced renal inflammation and fibrosis via a SNRK/NF-κB/p65 regulatory axis

Fig. 3

Reduction of miR-103a-3p ameliorates AngII‐induced renal injury. Mice (n = 8 per group) injected with LNA-anti-miR-103a-3p or LNA-anti-miR-control were infused with a vehicle control (Veh) or AngII (1 mg kg−1 d−1) for 4 weeks. a Serum miR-103a-3p levels were analyzed by qRT-PCR. b Kidney tissue miR-103a-3p levels. c Albumin excretion rate. d Albumin/creatinine ratio (ACR). e Serum creatinine levels. f Kidney weight to body weight ratio (KW/BW). g Western blot analysis of Mcp-1 and Tnf-α expression in the kidneys. h Representative images of trichrome staining and immunohistochemical staining of collagen type IV (Col IV) in the kidneys (scale bar = 50 µm). i Collagen type I and IV mRNA levels were measured using qRT-PCR. For all experiments, n = 8 per group; *p < 0.05 relative to vehicle control; #p < 0.05 relative to LNA-anti-miR-Control. Statistical analysis was carried out by a Student’s two-tailed t-test. The corresponding source data are available in the Source Data file

Back to article page