Fig. 4

SNRK is a target of miR-103a-3p. a Sequence analysis of human and mouse miR-103a-3p and SNRK mRNA 3′UTR. Putative miR-103a-3p and SNRK mRNA 3′UTR binding regions are highlighted with red boxes. b–e Primary GnECs were transfected with miR-103a-3p (b, c) or anti-miR-103a-3p (d and e) for 36 h. Snrk protein levels were assessed using Western blotting and densitometry (b, d), and Snrk mRNA abundance was measured using qRT-PCR (c, e). For all experiments, n = 6 per group; *p < 0.05 relative to miR-Control. f Luciferase activity assays were performed to assess whether the regulatory effect of miR-103a-3p requires the predicted binding sites in the SNRK mRNA 3′UTR in HEK293 cells. pMIR167 and pMIR1994 harbored the 167 and 1994 putative binding sites, respectively (indicated in a), whereas the respective binding sites were individually deleted in pMIR167∆ and pMIR1994∆. n = 6 per group, *p < 0.05 relative to pMIR; ^#p < 0.05 relative to pMIR-167; ^&p < 0.05 relative to pMIR-1994. Statistical analysis was carried out with a Student’s two-tailed t-test. The corresponding source data are available in the Source Data file