Fig. 4 | Nature Communications

Fig. 4

From: Hydro-Seq enables contamination-free high-throughput single-cell RNA-sequencing for circulating tumor cells

Fig. 4

Gene expression and clustering of breast CTCs. a–d Breast cancer therapy related genes: a human epidermal growth factor receptor 2 (HER2/Erbb2), b estrogen receptor (ESR1), c Androgen (AR), and d progesterone receptor (PGR). e–h MET-related genes: e E-cadherin (CDH1), f Epithelial Cell Adhesion Molecule (EPCAM), g Keratin-8 (KRT8), and h Keratin-18 (KRT18). i, j Cell cycle genes: i c-jun (JUN), and j cyclin D1 (CCND1). k–n EMT-related genes: k Phosphoinositide-dependent kinase-1 (PDK1), l Plasminogen activator inhibitor-1 (SERPINE1), m EMT transcription factor (ZEB2), and n transforming growth factor β (TGFB1). o The clustering and separation of HER2+ MET-like and HER2- EMT-like CTCs. p–u Stemness related genes: p CD44, q CD24, r vimentin (VIM), s pan-ALDH isoforms (ALDH), t ALDH1a3, and u CD90 (THY1). (Each dot represents one CTC. Green color represents the lowest expression, and red color represents the highest expression. The expression is logarithmically normalized. 666 CTCs from 21 patient samples were plotted based on tSNE clustering method.) Source data are provided as a Source Data file

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