Fig. 7
From: Dynamic assembly of ribbon synapses and circuit maintenance in a vertebrate sensory system
Light response properties and synaptic transmission in ribeye-ko mice. a (Left) Action potentials of ON-alpha RGCs evoked by a 500 ms light flash. (Right) Average firing rates during the stimulation duration. b (top) Whole-cell voltage-clamp recording of spontaneous excitatory postsynaptic currents (sEPSCs) from ctrl and KO. (bottom) Mean amplitude and frequency of sEPSCs. c Chirp stimulus (top) and example excitatory synaptic inputs to ON-alpha RGCs from ribeye knockout (red) and littermate control (black) retinas. d Increment and decrement responses from first section of chirp stimulus. The increment/decrement ratio determines how large the tonic excitatory input (i.e., the current suppressed by the decrement) is compared to the maximal excitatory input (i.e., the current in response to the increment). e Responses to frequency ramp. The amplitude of the response to each cycle of the stimulus is plotted against temporal frequency. Frequency tuning was measured from the frequency (F1/2) at which the response fell to half of its maximal value. f Responses to contrast ramp. The amplitude of the response to each cycle of the stimulus is plotted against contrast. Contrast sensitivity was measured from the contrast (C1/2) at which responses reached half maximum. g Summary of increment/decrement ratios (left), frequency tuning (middle) and contrast sensitivity (right) across cells. Low rod light level is 2 R*/rod/s, medium rod is 50 R*/rod/s and cone is 2000 R*/cone/s. *** denotes p < 0.001 (unpaired t-test). Data are presented as mean ± sem
