Fig. 7
Sequential association/dissociation of TFIIE and TFIIH. Once a stable TFIID–TFIIA–TFIIB–Pol II–TFIIF promoter complex is assembled, TFIIE and TFIIH can be recruited. The TFIIEβ subunit is required to anchor TFIIEα. Altgough the Core-TFIIH sub-complex can incorporate the complex in absence of TFIIE, TFIIEα, and TFIIEβ are both required to anchor the CAK within the PIC. In presence of ATP, Pol II phosphorylation is accompanied by the release of the CAK and TFIIEα from the promoter, a process that takes place before DNA opening. In presence of NTP, RNA synthesis is initiated, the Core-TFIIH and TFIIEβ are removed while DSIF and other elongation factors are recruited
