Fig. 3
From: Structural basis for substrate gripping and translocation by the ClpB AAA+ disaggregase
Substrate interactions by the NBD2. a Side view of the P3 NBD2 pore loop-substrate interactions with the D2 and D2′ loops indicated. b Map and model of P3 D2 pore loop showing arrangement of Y653 and V654, along the substrate density modeled with poly-Ala (yellow). c The D2 and D2′ pore loops of P2–P4, colored by protomer, and substrate (yellow), with distances between interacting residues and substrate backbone shown. d Schematic of D2 and D2′ loops interacting along the substrate for protomers P1–P5 with distances along the substrate axis shown based on the position of Y653 and H641 for the D2 and D2′, respectively. e ClpB, ClpBE639K, ClpBK640A, ClpBK640E, ClpBH641A, or ClpBH641E luciferase disaggregase activity was measured in the presence of KJE plus ATP. Values represent means ± SD (n = 2)
