Fig. 6

Conformational changes and nucleotide states of the protomers at the seam interface. a Post-process masked cryo-EM map and molecular model of protomers P1 and P6 in the “Pre” and “Post” conformational states following particle re-centering and focused refinement of the seam interface. b Protomers P1 and P6 for both Pre (gray) and Post (colored) states with substrate (yellow), shown superimposed following alignment to protomer P3 in the hexamer. Conformational changes are shown as distinct rotations (arrows) of the NBD1 and NBD2. c The Pre- (gray) and Post-state (colored) NBD1 and NBD2 pore loops shown superimposed for protomers P1 and P6 with distances indicated following alignment of P3 pore loops in the hexamer for both states. d Schematic showing proposed NBD1 and NBD2 nucleotide states of the hexamer for the Pre and Post states, colored to indicate ATP (green), ADP (red), and APO (gray) state, based on analysis of the nucleotide pockets in the two structures (Supplementary Fig. 6e, f). Protomers that are disconnected from the substrate are indicated with a dash and Arg-finger contact is indicated by the interlocking contact between two protomers