Fig. 2

In vitro evaluation of the NanoM1-BMP2 wound dressing cytotoxicity. MRC-5 cell line was cultured in the presence of Polyurethane film, (RM-A; a–e), NanoM1-BMP2 wound dressing (f–j) or high density polyethylene film (RM-C; k–q). Five sizes (20, 16, 9, 4, 1 mm²) of membranes were used. Cells were inspected for morphological changes over a 3 day period. Proliferation/viability was assessed via WST-1 assay (p–n) at 24 h (C.Ris Pharma, CRO, France). Error bars represent standard deviation. Scale bar: 100 µm