Fig. 2
From: The structural basis of translational control by eIF2 phosphorylation
eIF2α conformational flexibility on binding to eIF2B. a eIF2α conformation differs between eIF2αP/eIF2B and ternary complexes (TCs) complexes. eIF2α from our eIF2αP/eIF2B complex (domains 1 and 2 shown in gold and arrowed, domain 3 grey) aligned onto TC from 3JAP (2α domains 1–3 in grey) using eIF2α domain 3 as a reference. b–g Flexibility between eIF2α domains 2 and 3 seen in eIF2αP/eIF2B 3D classes obtained when halves of the particles were independently classified using a localised reconstruction script (described in the Methods section and Supplementary Fig. 2b). h eIF2αP conformations from b–g aligned modelled onto 3JAP eIF2 (as in a) as semi-transparent ribbons. Dashed black arrows indicate changed positions. In a, h, Ser52 and Ser52(P) side chains are shown in red
