Fig. 4 | Nature Communications

Fig. 4

From: The structural basis of translational control by eIF2 phosphorylation

Fig. 4

eIF2B antagonises RNA -activated protein kinase (PKR) activity. a Western blot of eIF2α phosphorylation (1 µM eIF2) by PKR (10 nM), when incubated in the presence of increasing concentrations of eIF2B. eIF2α and eIF2αP resolved by Phos-tag acrylamide gel electrophoresis. Both panels are from the same blot. Experiment repeated four times with similar results. Source data are provided in the Source Data file. b Quantification of eIF2αP percentage

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