Fig. 2 | Nature Communications

Fig. 2

From: Proteome-wide detection of S-nitrosylation targets and motifs using bioorthogonal cleavable-linker-based enrichment and switch technique

Fig. 2

Total Cys analysis by iodoTMT and Cys-BOOST. a Comparison of iodoTMT and Cys-BOOST workflows for total Cys peptides analysis from 100 μg of HeLa lysate per replicate. b and c pie charts showing the share of the target (Cys containing) and background peptides. d Boxplots of scaled log2 TMT reporter intensities of all quantified Cys containing peptides display a substantially more accurate and precise quantification for Cys-BOOST as compared with iodoTMT (center line: mean; box limits: upper and lower quartiles; whiskers: correspond to the highest or lowest respective value or if the lowest or highest value is an outlier it is 1.5 × interquartile range (IQR)). e Average TMT reporter intensities of Cys-containing PSMs (error bars show the minimum and maximum of n = 3 replicates) indicate a higher recovery by Cys-BOOST. f Number of Cys containing peptides quantified per μg of HeLa lysate. Source data are provided as a Source Data file

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