Fig. 5
From: Structural insights into substrate recognition by the SOCS2 E3 ubiquitin ligase
Position and characterization of SNPs of SOCS2. a Sequence conservation mapped onto the SH2 domain of SOCS2 (shown as surface). Conservation surface representation based upon the ClustalW multiple sequence alignment of CIS and SOCS1–SOCS7 sequences where highly conserved residues are shown in red/orange color and variable residue positions colored white/gold. Positions of SNP mutations are highlighted. b Transverse relaxation rates (R2) of spy molecule 3 binding to SBC protein variants. The R2 relaxation rates were obtained by fitting as exponential decay the 19F peak integrals of 3 measured at five CPMG delays in a sample in the absence or presence of SBC protein (see Supplementary Fig. 7). Error bars reflect the quality of the fit between the non-linear least-square curve and the experimental data
