Fig. 6
From: Structural insights into substrate recognition by the SOCS2 E3 ubiquitin ligase
Comparison of the GHR and EpoR binding. a Overlay of the GHR_pY595 (yellow) and EpoR (orange) peptides as bound to SOCS2. Side chains circled in blue have similar properties and red have different properties. b Different hydrophobic interactions (pink) caught by GHR_pY595 (yellow) and EpoR (orange) peptide. c Overlay of the substrate peptide of SOCS proteins. The GHR_pY595 (yellow) and EpoR (orange) peptides catch distinct hydrophobic cavities (pink) on SOCS2. These interactions differentiate SOCS2 substrate binding mode from other substrates of SOCS proteins. The SOCS3 substrate peptide gp130 is in cyan (PDB ID: 2HMH) and SOCS6 substrate peptide c-Kit is in green (PDB ID: 2VIF). d The binding of the non-canonical GHR (cyan) relies on backbone to backbone interaction and hydrophobic interaction from pY(+3) and pY(+5) of the GHR_pY595 to the SH2 domain hydrophobic core (pink). The binding mode of EpoR (orange) makes it unfavorable to establish these interactions
