Table 2 Affinity measurements for GHR_pY595 and EpoR_pY426 wild-type and mutant peptides binding to SBC
From: Structural insights into substrate recognition by the SOCS2 E3 ubiquitin ligase
Sequence | Peptide | SPR KD (μM) | NMR Ki (μM) |
|---|---|---|---|
PVPDpYTSIHIV | GHR wild-type | 1.5 ± 0.09 | 1.5 ± 0.06 |
PRPDpYTSIHIV | V(−3)R | 9.0 ± 1.05 | 20.6 ± 2.09 |
PYPDpYTSIHIV | V(−3)Y | 3.3 ± 0.13 | 3.3 ± 0.24 |
AVPDpYTSIHIV | pY(−4) | 1.7 ± 0.14 | 0.9 ± 0.17 |
PAPDpYTSIHIV | pY(−3) | 2.7 ± 0.19 | 3.4 ± 0.25 |
PVADpYTSIHIV | pY(−2) | 1.4 ± 0.12 | 4.1 ± 0.75 |
PVPApYTSIHIV | pY(−1) | 6.2 ± 0.37 | 21.7 ± 3.34 |
PVPDpYASIHIV | pY(+1) | 1.0 ± 0.09 | 0.5 ± 0.07 |
PVPDpYTAIHIV | pY(+2) | 1.4 ± 0.07 | 1.2 ± 0.06 |
PVPDpYTSAHIV | pY(+3) | 3.7 ± 0.22 | 3.4 ± 0.66 |
PVPDpYTSIAIV | pY(+4) | 6.7 ± 0.78 | 18.5 ± 2.67 |
PVPDpYTSIHAV | pY(+5) | 2.4 ± 0.13 | 1.4 ± 0.36 |
PVPDpYTSIHIA | pY(+6) | 1.9 ± 0.09 | 1.6 ± 0.21 |
ASFEpYTILDPS | EpoR wild-type | 13.3 ± 0.59 | 7.1 ± 1.01 |
AAFEpYTILDPS | pY(−3) | 14.2 ± 0.65 | 7.5 ± 1.04 |
ASAEpYTILDPS | pY(−2) | 16.3 ± 1.02 | 7.2 ± 0.42 |
ASFApYTILDPS | pY(−1) | 21.7 ± 1.49 | 23.5 ± 2.99 |
ASFEpYAILDPS | pY(+1) | 17.1 ± 0.38 | 10.3 ± 1.28 |
ASFEpYTALDPS | pY(+2) | 30.8 ± 1.24 | 17.2 ± 0.89 |
ASFEpYTIADPS | pY(+3) | 32.3 ± 1.42 | 20.4 ± 0.88 |
ASFEpYTILAPS | pY(+4) | 18.0 ± 0.91 | 14.1 ± 1.74 |
ASFEpYTILDAS | pY(+5) | 8.9 ± 1.25 | 6.9 ± 1.39 |
ASFEpYTILDPA | pY(+6) | 16.4 ± 0.29 | 11.3 ± 1.29 |
