Fig. 1

RSV accumulates a rich and distinctive protein corona in different biological fluids. a–c Principal component analyses (PCA) of the corona proteomic profiles of RSV, HSV-1, and controls. Triplicate samples were incubated with 10% v/v solutions of each different biological fluid for 1 h at 37 °C, then re-harvested, washed, and finally analyzed by MS. Only proteins significantly detected (FDR 1%) in all three replicates in each condition were used. NI = non-infected supernatant, (−)Lipo = negatively charged lipid vesicles, 200 nm, (+)Lipo = positively charged lipid vesicles, 200 nm. a PCA comparing proteomic profiles in human plasma (HP). b PCA comparing proteomic profiles in fetal bovine serum (FBS). c PCA comparing the corona profiles of RSV in different biological fluids; HP, FBS, MP, or BALF. d Heatmap representing the viral corona fingerprints of RSV after incubation in different biological fluids. The three columns in the heatmap show three replicates. Only proteins significantly detected (FDR 1%) in all three replicates in each condition were used. Red and blue indicate higher and lower than the mean protein signal, respectively. Scale bars represent row Z-scores