Fig. 5
From: The viral protein corona directs viral pathogenesis and amyloid aggregation
RSV protein corona in FBS and MP. a Serum-free produced RSV was pre-coated with different coronae via pre-incubation with different biological fluids prior to infection of HEp-2 cells in serum-free medium (diluted 10×) at an MOI of 1. The frequencies of GFP+ cells were quantified by flow cytometry 72 h post infection. Means ± SEM of six replicates from two separate experiments are shown. Significant differences in comparison with RSV (no corona) were assessed by non-parametric Kruskal–Wallis one-way ANOVA with Dunn’s multiple comparison test and are indicated by *P < 0.05, **P < 0.01, and ***P < 0.001, respectively. b Neutralization curves with different concentrations of palivizumab (monoclonal antibody targeting RSV F-protein) of RSV with or without FBS and MP protein coronae at the protein concentration of 0.3 mg/mL protein. Means ± SEM of six replicates from two separate experiments are shown. c The unique factors in FBS and MP from Fig. 4 are listed. d Gene list enrichment analysis of the total RSV corona profile in FBS and MP groups. Only proteins significantly detected (FDR 1%) in all three replicates in each condition were used. The top five enriched terms are shown in each GO domain
