Fig. 6

RSV accelerates the kinetics of amyloid formation. a NNFGAIL peptide incubated with ThT solution and RSV (3 × 10⁸ genome copy/mL), non-infected supernatant or serum-free viral production medium (VP-SF medium). ThT fluorescence was measured at 440 nm excitation and 480 nm emission over 12 h at 37 °C. Means ± SEM of six replicates from two separate experiments are shown. b, c Negatively stained TEM images of RSV incubated with 1 mM NFGAIL for 100 mins at 37 °C. b Fibrillar tangles are shown. Bar = 5 µm. c RSV virion shown at the base of an amyloid fiber. White arrows indicate viral particle and black arrows indicate fibrillar structures. Bar = 500 nm. d NNFGAIL peptide incubated and monitored at similar conditions in the presence or absence of 5% FBS, lipid vesicles (positively or negatively charged, dimeter = 200 nm, concentration = 1 × 10¹⁰ particles/mL), or VP-SF medium. Means ± SEM of six replicates from two separate experiments are shown. e GNNQQNY peptide incubated with RSV, non-infected supernatant, or VP-SF medium. Means ± SEM of six replicates from two separate experiments are shown