Fig. 1

Dynamic distribution of 6mA in Drosophila early embryo genomes. a Timeline of the early embryogenesis and the 6mA-DNA-IP-Seq experimental process. The cleavage cycle is described according to a previous study²⁵. DNA samples were collected from 0.75-h (nearly pre-MZT and pre-ZGA), 3-h (post-ZGA), and 6-h (post-MZT) post-fertilization embryos. b Overlap of 6mA enrichment peaks in 0.75-, 3-, and 6-h stage embryos. In cases where one peak in one sample overlapped multiple peaks in another sample, we selected the overlapped peak number from one sample as representative. c The average 6mA signal profiles for the common peaks in 0.75-, 3-, and 6-h stage embryos. d The average 6mA signal profiles in 0.75- and 3-h unique peaks and their common peaks. e Examples of 6mA-marked regions in which the 6mA modification signals were dynamically changed in early embryos. f Overlap of 6mA peaks identified in 0.75-, 3- and 6-h of wild type and 6-h DMAD knockdown (KD) samples. g The average 6mA signal profiles in peaks gained in 6-h DMAD knockdown samples, when compared with 6-h wild-type samples. These peaks were also detected in samples at 0.75-h or 3-h stages. h An example of 6mA signal in 0.75-, 3- and 6-h of wild-type stage and 6-h DMAD knockdown samples. MZT maternal-to-zygotic transition, ZGA zygotic genome activation