Fig. 4

Jumu functions as a maternal factor to regulate embryogenesis. a Western blot assays showing Jumu protein abundance in ovary, unfertilized eggs and embryos at various embryonic stages as indicated. b Two jumu mutant alleles, jumu¹ and jumu², were generated by the CRISPR/Cas9 system. c, d Western blot assays were performed to show expression levels of Jumu protein in wild type or jumu mutant ovaries (c), and in wild type or maternal jumu mutant eggs (d). e Relative hatching rate of embryos with indicated genotypes. The two-tailed Student’s t test was used to analyze statistical variance. Data expressed as means of 3 independent experiments. Error bars indicate mean ± s.d. ***P < 0.001. f Three typical phenotypes were observed in jumu maternal lethal embryos (M⁻, Z⁺, jumu). g Wild type and maternal jumu mutant embryos were stained with phalloidin and Hoechst to visualize F-actin (red) and DNA (blue), respectively. Scale bar, 10 μm. Source data are provided as a Source Data file