Fig. 1

Bcl10 disruption in mature regulatory T cells (Tregs) results in autoimmune inflammation. a Quantified analysis of the total numbers of viable splenic CD4⁺Foxp3⁺ Tregs of 16-day-old male Bcl10^fl/fl;FIC or Bcl10^fl/fl control mice. Data are cumulative from four independent experiments. b Histological hematoxylin and eosin (H.E.) staining of the indicated organs on day 25 post-partum. The black bar in the lower right corner depicts the scale of 50 μm. Pictures are representative of ≥2 mice per genotype. c Survival curves of male Bcl10^fl/fl or Bcl10^+/fl;FIC control mice (black line, n = 10) versus Bcl10^fl/fl;FIC mice (red line, n = 14). Survival equals the day the animal had to be sacrificed to avoid severe burden. Statistical significance between the survival curves with the corresponding P value was calculated by a log-rank (Mantel–Cox) test. d Concentration of indicated inflammatory cytokines in the sera of 16-day-old male Bcl10^fl/fl (dots) and Bcl10^fl/fl;FIC (squares) mice. Each dot or square represents one mouse. e Frequency of CD4⁺Foxp3^– (upper panel) and CD8⁺ (lower panel) CD44^hiCD62L^lo effector T cells (T_eff) in the spleens of 16-day-old male Bcl10^fl/fl and Bcl10^fl/fl;FIC mice as measured by fluorescence-activated cell sorting (FACS). Data are cumulative from seven or three independent experiments. f Median fluorescence intensity (MFI) of cell surface CD86 (left) and MHCII (right) on CD19⁺B220⁺-gated splenic B cells of 16-day-old male Bcl10^fl/fl and Bcl10^fl/fl;FIC mice. Data are cumulative from four or three independent experiments. g Quantification of anti-cardiolipin (left) and anti-dsDNA immunoglobulin (right) concentrations in the sera of 16-day-old male Bcl10^fl/fl and Bcl10^fl/fl;FIC mice. The bars in a and d–g represent the mean ± SD; statistical significance between Bcl10^fl/fl and Bcl10^fl/fl;FIC mice was assessed by a two-tailed unpaired Student’s t test. Significance values are depicted in the graph; (ns) not significant. Source data are provided as a Source Data File