Fig. 2

BCL10 signaling controls the homeostatic resting regulatory T cell (rTreg) to effector Treg (eTreg) conversion. a Survival curves of Bcl10^fl/fl;FIC (black line, n = 4) versus Bcl10^fl/fl;FIC/FIC female mice (red line, n = 6). P value was calculated by a log-rank (Mantel–Cox) test. b Fluorescence-activated cell sorting (FACS) profiles to detect either EYFP^– (left) or EYFP⁺ (right) CD62L^hi naive rTregs and CD44^hiCD62L^lo eTregs in the viable CD4⁺Foxp3⁺ splenic Treg population of Bcl10^+/+Rosa26^LSL-EYFP;FIC (left plots) and Bcl10^fl/flRosa26^LSL-EYFP;FIC (right plots) female mice. Plots are representative of four mice each. c Frequencies of splenic EYFP^–- and EYFP⁺-gated CD62L^hi naive rTregs (upper panel) and CD44^hiCD62L^lo eTregs (lower panel) in the viable CD4⁺Foxp3⁺ Treg population of female Bcl10^+/+;Rosa26^LSL-EYFP;FIC and Bcl10^fl/fl;Rosa26^LSL-EYFP;FIC mice. Statistical significances were assessed by ordinary one-way analysis of variance (ANOVA) combined with Tukey’s multiple comparisons test. d Representative FACS experiment to detect the differentiation of sorted splenic CD4⁺EYFP⁺CD62L^hi naive rTregs of ≥2 pooled female Bcl10^fl/fl;Rosa26^LSL-EYFP;FIC and Bcl10^+/fl;Rosa26^LSL-EYFP;FIC control mice into CD4⁺EYFP⁺CD44^hiCD62L^lo eTregs following 3 days of stimulation with anti-CD3/CD28. e Quantified percentages of differentiated eTregs. Data are from four independent experiments with ≥2 pooled mice per genotype. To avoid prominent effects of single data points on the mean, statistical significance was assessed by a two-tailed ratio-paired t test with corresponding paired data points of one experiment connected by a line. f FACS analysis to detect viable splenic CD4⁺Foxp3⁺ Tregs in 12-week-old FIC (n = 4) or Rosa26^{LSL-CARD11-CA};FIC female mice (n = 5). g Frequency of viable splenic CD4⁺Foxp3⁺ Tregs in 12-week-old FIC or Rosa26^{LSL-CARD11-CA};FIC female mice. h Representative FACS profile detecting the percentages of CD62L^hi rTregs and CD44^hiCD62L^lo eTregs within the viable CD4⁺Foxp3⁺ splenic Treg population of 12-week-old FIC (n = 4) or Rosa26^{LSL-CARD11-CA};FIC (n = 5) female mice. i Frequencies of viable splenic CD44^hiCD62L^lo eTregs in FIC control or Rosa26^{LSL-CARD11-CA};FIC female mice. To detect the transgene in h, i, gating was performed on CD4⁺Foxp3⁺GFP⁺ cells. Statistical significances in g, i were assessed by a two-tailed unpaired Student’s t test. Bars in c, e, g, i represent the mean ± SD. Data in c are representative of three independent experiments, while data in f–i are cumulative from two independent experiments. Source data are provided as a Source Data File