Fig. 3 | Nature Communications

Fig. 3

From: Recapitulation of HDV infection in a fully permissive hepatoma cell line allows efficient drug evaluation

Fig. 3

Long-term infection and progeny secretion. HepNB2.7 cells were inoculated with HDV at 0.2/0.6 or 2 IU/cell. The cell supernatant was collected every third day starting from day 3 post infection until day 33. a HBsAg in the supernatant was quantified by ELISA. b Total RNA was extracted from the cell supernatant and HDV RNA was quantified by RT-qPCR. c The supernatant of the respective time points was used as an inoculum for a secondary infection on HuH7-NTCP cells. HDAg-positive cells of the secondary infection were quantified by automated image analysis

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