Fig. 1
From: Model-based understanding of single-cell CRISPR screening
General workflow of MUSIC. MUSIC comprises three steps for single-cell CRISPR screening data analysis: data preprocessing, model building, perturbation effect prioritizing. In the 1st step, besides the conventional considering of cell quality, several specific factors existed for single-cell CRISPR screening are also considered. These factors are the ratio of nonzero perturbed expression value in all cells, sgRNA efficiency and the minimal perturbed cell number per perturbation. In the 2nd step, MUSIC applies a topic model-based computational framework to derive the functional topics of each cell (including controls) with specific perturbation (PE, perturbation). In the 3rd step, MUSIC quantitatively estimates and prioritizes the individual gene perturbation effect on cell phenotypes from three different perspectives, i.e., prioritizing the gene perturbation effect as an overall perturbation effect, or in a functional topic-specific way, and quantifying the relationships between different perturbations
