Fig. 2
From: Airway response to respiratory syncytial virus has incidental antibacterial effects
Analysis of the airway proteome by tandem mass spectrometry. Analysis of airway proteomes was undertaken using mass spectrometry in a population of 40 children with RSV infection and 44 RSV-negative children. a Differences in the mean expression levels of local airway proteins between the RSV-positive and RSV-negative children was analysed using two-sided Student's t-tests and presented in a volcano plot. The y-axis represents −log10 fdr-adjusted P values (q values) computed using two-sided Student's t-tests and the x-axis represents −log10 fold change in protein expression. Red circles represent differentially expressed proteins (defined by q values: ≤0.05 and ≥1.5-fold change in expression between groups). Red circles in the top left and top right quadrants represent proteins that were significantly elevated in RSV-positive and RSV-negative children, respectively. b, c Gene ontology (GO)-based biological pathways analysis was used to identify biological processes that were significantly overrepresented in RSV-positive and RSV-negative children. Those that were significantly overrepresented in RSV-positive children are shown in b while those that were overrepresented in RSV-negative children are shown in c. Adjusted P-values in b and c were calculated on the enrichR platform and are based on the Fishers extract test. The most significantly enriched pathway in RSV-positive children was neutrophil degranulation and in RSV-negative children was SRP-dependent co-translational protein targeting to membrane, signal sequence recognition. d, e The specific proteins that were significantly differentially expressed within those pathways are shown in d and e, respectively. The proteins highlighted with the red boxes in d are neutrophil granule proteins. The elements of the box and whisker plots are defined in Fig. 1. Source data are provided as a Source Data file
