Fig. 5

DVL2 orchestrates the recruitment of CEP192 at protrusions. a, c MDA-MB-231 cells were transfected with control, CEP192 or DVL2 siRNAs for 72 h, and then stimulated overnight with DMEM or ACM. Cells were stained for (a) CEP192 and actin, or (c) CEP192 and PCNT. Bar = 20 µm. b, d, e Intensity of CEP192 at cell cortex (b), at centrosome (d), and PCNT signal at centrosome (e) was measured as described in Methods and plotted as box and whiskers. Boxes represent median and 25th to 75th percentiles, whiskers the minimum and maximum values with each individual value superimposed. The data were compared with one-way ANOVA Kruskal–Wallis test and post-tested with Dunn’s multiple comparison test. Arrow indicates the control bar used for comparison. (*p < 0.05, ****p < 0.0001; n = 3, at least 60 cells were measured per condition). Light blue is CEP192, light green is PCNT. f Model for regulation of centrosomal and cortical CEP192 pools by DVL2