Fig. 5
From: Iminothioindoxyl as a molecular photoswitch with 100 nm band separation in the visible range
Spectroscopy studies on the substituent effects on ITI photo-isomerization. a Absorption spectra of 40 μM ITIs 1a–f in MeOH. b Transient absorption spectra of ITIs 1a–f in MeOH after irradiation at 430 nm after 3 ms delay. c Transient absorption spectroscopy of 120 μM p-MeO-ITI 1b in aqueous PBS buffer (6.7% DMSO), irradiated with a 10 ns 430 nm light pulse and spectra recorded with 1 ms delay steps. The purple line indicates the absorption spectrum of 120 μM Z-ITI 1b in aqueous PBS buffer (6.7% DMSO). The color bar represents increased delay in transient absorption spectroscopy. d Cuvettes 1 and 2 contain 200 µM ITI 1b in MeOH. Left: both thermally adapted. Middle: cuvette 2 irradiated with 400 nm light while cooled at −60 °C in acetone bath. Right: reheating of cuvette 2 to room temperature. e Three cycles of photo-isomerisation of 100 µM 1b in MeOH, thermally adapted and switched with 400 nm light, while cooled at −60 °C in acetone bath (Supplementary Fig. 89)
