Fig. 2

Blimp1-YFP⁺ cell-derived organoids express sebaceous gland markers. a Schematic view of the sebaceous gland structure and various cell type markers. b Tail skin wholemount extracted from P56 (telogenic)-old wild type (WT) mouse stained for Keratin-15 (K-15) and Ki67. c, d Confocal analysis of Blimp1-YFP⁺ generated organoids cultured for 10 days, stained for (c) Ki67 and (d) MCM2, showing proliferating cells in the outer layer. e Confocal analysis of Blimp1-YFP⁺-generated organoids cultured for 10 days, stained for K-15 and K-5. f–h BrdU pulse-chase analysis for (f) 24 h, (g) 48 h, and (h) 72 h showing migration of BrdU-labeled cells from the outer layer to the inner non-proliferating mass. i Representative time points of live imaging of movement kinetics, utilizing light sheet fluorescence microscopy, examining 7-day-old organoids derived from Blimp1-YFP-H2B-GFP⁺ (nuclear labeled) cells over a 24 h period. Two tracked cells and their progeny (blue and red dots) over time are marked. All images are representative of at least n = 3 mice. In vitro experiments were repeated at least three times with similar results. Organoids were grown from a mix of at least three mice and cultured in three independent biological repeats. Scale bars: 10 μm (i), 20 μm (b–e), 50 μm (f–h)