Fig. 2
From: Contemporaneous 3D characterization of acute and chronic myocardial I/R injury and response
High resolution light sheet fluorescence microscopy (LSFM) of murine and human tissue. a 3D reconstruction of the inferior (dorsal) half of an ECi-cleared murine heart depicting both CD31 (blood vessels; green) and autofluorescence (total heart tissue; grey) showing distinct heart structures such as right ventricular wall (RVW), right ventricle (RV), intraventricular septum (IVS), left ventricle (LV), left ventricular wall (LVW) and left atrial appendage (LAA). b Single optical slice obtained by LSFM showing a cross-section of the LVW (enlarged ROI highlighted in a) with cellular detail using autofluorescence (grey) only. Enlargements show that high autofluorescence in restricted regions is of cellular origin (ROI left, different contrast settings) and that the directionality of visualized cardiac cells depends on their respective localization within the muscle (ROI right). Magnification: 8 × . c 3D reconstruction of the aortic valve in a top-view visualized by autofluorescence (grey) and CD31 (green). Magnification: 4 × . d BALANCE applied to murine liver enhances sample transparency (left) and enables imaging of large tissue samples for quantitative purposes (mid and right). High contrast between vessel lumen and surrounding tissue allows direct rendering of the portal vein system from autofluorescence raw data (mid). e Left: BALANCE also enables clearing of a human left atrial appendage (LAA) biopsy and enhances sample transparency. Mid: The autofluorescence signal alone gives a structural overview and single cellular resolution (right). Scale bar values in µm. One square on the macroscopic images equals 2 × 2 mm. (ECi - ethyl cinnamate)
