Fig. 4

Correlation of echocardiography, cardiac troponin I (cTNI) plasma levels, triphenyl tetrazolium chloride negative (TTC^neg) areas and CD31 negative (CD31^neg) volumes after 24 h of reperfusion in the same 5 mice. a Top panel: Correlation of CD31^neg volumes and TTC^neg areas per slice (left) and heart (right) of various infarct sizes (left: n = 40 slices from 8 hearts; right: n = 8 hearts; r- and p-values depicted). Bottom panel: Associated Bland-Altman analyses of CD31^neg volumes and TTC^neg areas per slice (left, −1.08% mean difference) and per heart (right, −0.75% mean difference) of correlations depicted above (dashed black lines mark 95% confidence intervals, solid black line marks zero bias, red dashed line marks mean difference). b Inter-operator CD31^neg volume quantification variances. (Top) Absolute quantified volume of 3 users in 5 randomly selected slices of different hearts subject to 45 min of ischemia and 24 h of reperfusion. (Bottom) The quantification results per user relative to the average (100%, dashed line) quantified volume (in comparison to left, slice #4 was excluded as no CD31^neg volume was measurable). c Original micrographs of echocardiographic imaging. Dashed lines indicate left ventricular lumen. White stars indicate zones of abnormal left ventricular wall movement. d Same I/R injury heart as in c: exemplary TTC staining and subsequent light sheet fluorescence microscopy (LSFM)-based 3D reconstruction of the same slice. e Correlation of ejection fraction (EF) reduction with TTC^neg areas and CD31^neg volumes, respectively, per heart (n = 5 hearts; r- and p-values depicted). f Left: raw data used for correlation of EF reduction, TTC^neg and CD31^neg with plasma cTNI levels (value for each animal given, n = 5). Right: correlation of absolute cTNI levels with EF reduction, TTC^neg and CD31^neg. Scale bar values in µm. Source data are provided as a Source Data file