Fig. 7

Characterization of immune cell infiltrates into I/R injury. a Example maximum intensity projections (MIPs) of Ly-6G positive (Ly-6G^pos) neutrophils after 24 h (left) as well as Ly-6G^pos neutrophils and F4/80 positive (F4/80^pos) macrophages after 5 d (middle; right). Depicted are CD31 (green, top row) and Ly-6G (turquoise) or F4/80 (purple) signals (middle row) together with a merge (bottom). Dashed white line marks the border of the CD31^curly region. Magnification: ×3.2 (24 h), 4 × (5 days). b Immune cell quantities (top neutrophils, bottom macrophages) determined by flow cytometry (white, filled dots) or based on LSFM (white, empty dots) analysis after 24 h and 5 d of reperfusion, as well as under baseline condition (n = 4–5; median ± interquartile range; Kruskal–Wallis test with Dunn’s correction). c Representative 3D visualization of Ly-6G^pos cell distribution across whole murine hearts. Heart structure (grey,) and knot (white) are shown for orientation. Ly-6G^pos cell populations are colored in respect to their localization in either AAR (red), I/R injury (yellow), CD31^curly (green) or outside the above-mentioned structures (turquoise). d Relative cell density ± 30 µm from the respective volume border (n = 5; median; Kruskal–Wallis test with Dunn’s correction). e 3D visualization of F4/80^pos signals depicted as a surface rendering (purple) in relation to heart surface (left; heart volume in grey) or CD31^curly surface (right; CD31^curly in green) after 5 d of reperfusion. The knot (white) is visualized for orientation. Scale bar values in µm. This finding was verified in 5 mice. Source data are provided as a Source Data file