Fig. 6
Sgf73p promotes export of specific transcripts during heat shock. a–c FISH analyses against (a) stress-inducible PHSP12 HSP12, (b) stress-inducible PHSP12 CYC1, and (c) non-inducible PCYC1 HSP12 genes were performed using Cy3-labeled specific probes. DNA was counterstained with DAPI. Scale bar, 1 μm. d ChIP-qPCR analysis against Sgf73p (α-FLAG) in mock and wildtype cells after heat shock, regarding a stress-inducible promoter (left and middle panel) and a non-inducible promoter (right panel). e Average plot of Mex67-5xFLAG enrichment in wildtype, rpt2-1, and sgf73Δ cells with respect to the Hsf1-dependent gene set (n = 18) before and after heat shock. Standard deviations obtained from three independent experiments are shown by error bars and P-values were determined by Student’s t-test (*P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001)
