Fig. 1

MnSOD^K68Q expression promotes a transformation-permissive phenotype in vitro. a Immortalization, i.e., growth beyond 15 passages, of pMEFs infected with lenti-MnSOD^WT, lenti-MnSOD^K68R, and lenti-MnSOD^K68Q and either lenti-Myc or lenti-Ras. b The cell lines above were tested for soft agar growth (upper) and colony formation (lower panels). c pMEFs infected with Ras^G12V were tested for immortalization, doubling time, and soft agar growth. d NIH 3T3 cells expressing MnSOD^WT, MnSOD^K68R, and MnSOD^K68Q were tested for growth in soft agar (upper) and colony formation (lower panels). Experiments done in triplicate. Scale bar: 20 μm