Fig. 5

MnSOD^K68Q expression leads to oxidative stress in human breast cells. a, b MnSOD activity: a MCF7-MnSOD^WT, MCF7-MnSOD^K68R, and MCF7-MnSOD^K68Q; and b T47D-MnSOD^WT, T47D-MnSOD^K68R, and T47D-MnSOD^K68Q in whole-cell homogenates. c, d Steady-state levels of O₂^●− were measured in c MCF7-MnSOD^WT, MCF7-MnSOD^K68R, and MCF7-MnSOD^K68Q and d T47D-MnSOD^WT, T47D-MnSOD^K68R, and T47D-MnSOD^K68Q cells. e, f H₂O₂ levels was measured in these cells by CDCFH₂ oxidation via flow cytometry. g, h Glutathione levels were measured in whole-cell homogenates of these cells. All experiments were done in triplicate. Error bars represent ± 1 SEM. *p < 0.05, **p < 0.01, and ***p < 0.001. A one-way ANOVA statistical analysis with Tukey’s post-analysis was used