Fig. 5

Peptides form amyloid structures and seed in vivo. a After 96 h of in vitro incubation, peptides from previous ThT experiments (Fig. 4c) were transduced into tau biosensor cells via lipofectamine (Methods). FRET signal from each condition (tau RD-CFP/tau RD-YFP) was measured by flow cytometry on three biological triplicates of at least 10,000 cells per condition. Error bars represent a 95% CI of each condition. Solid and dashed horizontal lines represent the mean and 95% error from untreated biosensor cells, respectively, for ease of statistical comparison. b–h Electron microscopy images of each peptide from previous ThT experiments (Fig. 4c). The black bar represents 200 nm distance in each image. i–p Qualitative fluorescence microscopy images of tau biosensor cells immediately prior to flow cytometry experiments. i shows a representative image of untreated biosensor cells. j–p each shows a representative image of biosensor cells treated with samples from b–h, respectively. The white bar represents 10 μm distance in each image