Fig. 1
DV activates platelets via CLEC2 to enhance NET formation. a Human platelets were pre-incubated with anti-CLEC2 mAb or isotype control mAb at room temperature for 15 min, followed by incubation with DV (PL046) at 37 °C for 1 h before subjected to flow cytometry analysis. b Platelets from WT and clec2−/− mice were incubated with DV (NCG-N strain) or aggretin for 1 h at 37 °C, followed by flow cytometry analysis. Data are presented as fold change in MFI over mock control. c Neutrophils were co-incubated with autologous platelets and DV for 3 h at 37 °C. DNA, histone, and MPO were detected by Hoechst 33342 (blue), anti-histone antibody (green), and anti-MPO antibody (red), respectively. d Human platelets were preincubated with isotype control or anti-CLEC2 mAb for 15 min before DV stimulation. e Mouse neutrophils were incubated with WT or clec2−/− platelets and stimulated with DV (NGC-N) or aggretin at 37 °C for 3 h. NET area was measured by immunofluorescence staining to determine the histone area (μm2). Data are mean ± SEM from at least three independent experiments. **p < 0.01, ***p < 0.001 (Student’s t-test). Scale bar: 10 μm. Source data are provided as Source Data file
